Sequencing of oligonucleotide phosphorothioates based on solid- supported desulfurization

نویسندگان

  • T. K. Wyrzykiewicz
  • D. L. Cole
چکیده

We described a solid-supported desulfurization procedure allowing easy access to the sequence analysis of oligonucleotide phosphorothioates. The described method is based upon selective removal of the 2-cyanoethyl phosphate protecting groups, followed by iodine-promoted desulfurization of the resulting phosphorothioate diesters. Automatic oxidation of oligonucleotide phosphorothioates, anchored via an ester linkage to a standard solid support (LCAA/CPG), is combined with Maxam-Gilbert solid-support sequencing. The overall procedure allows rapid simultaneous sequence analysis of several oligonucleotide analogs.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

DNA Fingerprinting Based on Repetitive Sequences of Iranian Indigenous Lactobacilli Species by (GTG)5- REP-PCR

Background and Objective: The use of lactobacilli as probiotics requires the application of accurate and reliable methods for the detection and identification of bacteria at the strain level. Repetitive sequence-based polymerase chain reaction (rep-PCR), a DNA fingerprinting technique, has been successfully used as a powerful molecular typing method to determine taxonomic and phylogenetic relat...

متن کامل

Study of the Desulfurization Process and Gas-Solid-Liquid Phase Distribution under the Complex Humidification Conditions in Dense Tower

In making the gas-solid-liquid phase analysis, based on the collaborative study of the internal and external humidification ratio of desulfurization efficiency, determine the humidifier ratio within 60% to 75%, that can make the best balance between the respective merits of a simple tower humidifiers tower humidifier way, optimizing the overall desulfurization reaction, effectively reducing the...

متن کامل

Specific inhibition of human immunodeficiency virus type 1 replication by antisense oligonucleotides: an in vitro model for treatment.

We have developed a culture system, simulating in vivo conditions of human immunodeficiency virus type 1 (HIV-1) infection, to evaluate the long-term efficacy of antisense oligonucleotide treatment. Five oligonucleotide phosphorothioates (28-mers), complementary to different regions of HIV-1 RNA, blocked replication of the virus in a sequence-specific manner at 1 microM concentration. Variation...

متن کامل

Effects of Modified Pyrolysis Tar on Gas Desulphurization Performance

The paper introducedeffects of modified pyrolysis tar on flue gas desulfurization. This experiment selected the pyrolysis tar as the raw material, researched the effects on desulfurization performance under different modification solution, concentration, solid liquid ratio of pyrolysis tar and modified solution, calcination temperature and calcination time by 16 group orth...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Nucleic acids research

دوره 22 13  شماره 

صفحات  -

تاریخ انتشار 1994